Challenge:
Solubilizing and stabilizing your membrane protein is always the primary challenge. Detergents usually pay a role in overcoming this task, but using detergents can create a whole new query…..What to do with the excess detergent left in the solution?
Removal of excess detergents usually means adding additional, complicated steps to your purification protocol, including dialysis and/or chromatography methods (e.g. ion exchange, SEC).
The DetEx Cation Mini Spin Column Solution:
The DetEx Cation Mini Spin Column is designed to rapidly and effectively remove excess detergents using weak cation exchangers. It is simple to use and requires only a micro centrifuge.
- Optimized for membrane proteins with pl > 6.0
- Complete detergent exchange/removal
- Fast -- removal and exchange of free detergent micelles in 10 minutes
- Generates concentrated protein free of detergent micelles
Applications:
1. Used in the final purification step prior to crystallization trials to ensure that free detergent micelles are removed from the sample. Removal of excess detergents has been shown to increase the likelihood of protein crystallization.
2. It is a simple method for small scale detergent exchange. The DetEx Columns allow for quick and easy exchange of membrane proteins into new detergents for crystallization trials.
Product Features:
- Weak cation exchanger for binding membrane proteins with pI > 6.0
- Column bed volume: 0.2 ml
- Max sample loading volume: 0.4 ml
- Typical protein binding capacity: 2 mg
- Elution in a small volume (minimum volume 50 μl)
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