Anatrace

Here you’ll find answers to researchers’ most frequently asked questions. If you have a question you’d like to submit, please send us an email at info@anatrace.com. And be sure to check this section often for new questions and answers.

What is the difference between ANAGRADE® and SOL-GRADE® detergents?
SOL-GRADE detergents are 97% pure by HPLC and are often used for membrane protein extraction from crude cell lysates. ANAGRADE® detergents are 99% pure by HPLC and are often used for protein purification and characterization. ANAGRADE® detergents are also low in UV absorbance and fluorescence. (See our Tech bulletin 102 Selecting the Grade of Detergent that is Right for your Application for more.)
What is the shelf life of your detergents?
All of our detergents were formulated to remain stable for extended periods of time. Several detergents (i.e., CHAPS, CYMAL®-3, HEGA®-10, FOS-CHOLINE®-10) were analyzed for stability by storing them at 40ºC for one week and at room temperature for two weeks. After this time period, no deterioration was noted and all detergents remained within specifications. Several other detergents (i.e., tetradecyl maltoside, dodecyl maltoside, and HEGA® -10) were heated to 55ºC in solution for two hours. These detergents remained within specifications after the heating cycle and had not deteriorated. (See our Tech bulletin 103 “Detergent Stability” for more.)
How should detergents be stored?
Our ANAPOE® detergents should be stored refrigerated in the dark. All other detergents should be stored frozen and dry. All detergents should be warmed to room temperature before opening the container. (See our Tech bulletin 103 “Detergent Stability” and Tech bulletin 107 “Detergent Analysis” for more.)
What methods does Anatrace, Inc. use to analyze detergents?
Our detergents are analyzed by HPLC using a standard C18 column in conjunction with a refractive index detector. Elutants include acetonitrile/water or methanol/water. Detergents are also analyzed by UV absorbance, fluorescence, conductance, solubility in water, pH, alcohol contamination, and percentage of alpha isomer (glucosides and maltosides). (See our Tech bulletin 102 Selecting the Grade of Detergent that is Right for your Application for more.)
What is the difference between the ANAPOE® detergents and the other polyoxyethylene detergents (i.e., Triton® X-100) listed in the catalogue?
ANAPOE® detergents are purified to remove peroxides that may form as polyoxyethylene detergents age. This makes ANAPOE® detergents oxidant-free, so they may be used to extract membrane proteins with a reduced risk of oxidizing sulfhydral groups.
Do you provide an MSDS and Certificate of Analysis with your product shipments?
Yes, we provide this information with each shipment. It can also be requested through this website, by email, or by contacting technical support.
Do you offer bulk pricing discounts?
Yes, we typically offer bulk pricing for quantities 4X the largest size listed in our catalogue. If you would like a written quote, please call 800-252-1280 or email your request to info@anatrace.com. Bulk pricing is applied to individual products – we will not discount orders based on the total size of the order.
Is there a difference between the terms “maltosides” and “maltopyranoside”?
These terms are used interchangeably for the same class of compounds. Likewise, glucoside and glucopyranoside refer to the same structure.
Do you have CAS Registry numbers for your detergents?
Yes, these numbers can be found in the “Registry Number Index” in our catalogue, and on this website.
What is the most cost-effective approach to determine the “right” detergent for my application?
We offer a variety of solid- and solution-based detergent kits, at a substantial discount, that can help you determine the best detergent for your needs. Our “Popular Detergent Kit” contains a variety of ionic and nonionic detergents.
Are your detergents soluble in water?
Many of our detergents are soluble to 10-20% in water at 0-5ºC. Maltosides are generally more soluble than glucosides. Detergents can be briefly heated to 50-60ºC, with stirring, to promote solubilization.
Are your detergents safe for human consumption?
Anatrace products are supplied for laboratory research only.
Do you list CMCs and aggregation numbers for your detergents?
Most of our products have CMC values, while some have aggregation numbers. You can find these numbers in the Technical Data section of our catalogue and in the product section of this website.
What do you mean by: “detergents are supplied as a 10% w/v solution”?
A 10% w/v solution consists of 10 g of the specific product dissolved in 100 mL of solution.
How does CMC relate to chain length?
As acyl chain length increases, the CMC decreases.