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In December of 2010, Pil Seok Chae and coworkers developed a new class of neopentyl glycol (NG) detergents aimed at improving the solubilization, stabilization, and crystallization of integral membrane proteins(1). Officially launched by Anatrace in February, 2011, these eight new detergents (specifically LMNG and OGNG) are gaining in popularity and have been used in the structure determination of a number of high-impact membrane proteins. These proteins cover a wide range of functional classes, including GPCR’s, channels, and transporters.
Of the 29 GPCR structures published since our 2011 release of the NG detergents, eight of these structures have relied on the use of LMNG for crystallization, typically in combination with CHS. These GPCR structures include the recently published OX2 orexin receptor bound to the insomnia drug suvorexant(2) (LMNG), and the Rhodopsin-Arrestin complex determined by LCP-SFX(3) (LMNG/CHS). Other non-GPCR membrane protein structures determined using neopentyl glycol detergents in 2015 include the ABC transporter PglK(4) (LMNG), the MFS transporter Ferroportin(5) (LMNG), the two-pore potassium channel TREK-2(6) (OGNG/CHS), and the Cryo-EM structure of the TRPA1 ion channel(7) (LMNG).
When selecting a detergent for crystallization, oftentimes very slight modifications to the detergent structure can have a huge effect. An example of this was observed in the structure of the CaaX protease Ste24p where the small change from C12E8 to C12E7 led to an improvement of the diffraction quality of the crystals(8). A common request from our customers was for an intermediate detergent between the 12 carbon chain length of Lauryl-Maltose Neopentyl Glycol (LMNG) and the 10 carbon chain length of Decyl Maltose Neopentyl Glycol (DMNG). You ask, we deliver! We are happy to launch the newest member of the neopentyl glycol family, Undecyl Maltose Neopentyl Glycol (UMNG), which serves as the perfect intermediate molecule between LMNG and DMNG.
But that’s not all! We have more exciting product launches coming in the next few weeks, including two new maltose-based MonoPod Amphipiles (MPA), three trehalose-based non-ionic detergents (DDT), and a more soluble version of Cholesterol Hemisuccinate (CHS-BisTris). To see information on these new detergents and our other new products coming soon, check out our new Coming Soon page.
References:
1) Chae, P. S., et al. (2010) Nature Methods 7(12), 1003-1008.
2) Yin, J., et al. (2015) Nature 519(7542), 247-250. PDB: 4S0V.
3) Kang, Y., et al. (2015) Nature 523(7562), 561-567. PDB: 4ZWJ.
4) Perez, C., et al. (2015) Nature 524(7566), 433-438. PDB: 5C78.
5) Taniguchi, R., et al. (2015) Nature Communications Oct 13(6), 8545. PDB: 5AYN.
6) Dong, Y. Y., et al. (2015) Science 347(6227), 1256-1259. PDB: 4BW5.
7) Paulsen, C. E., et al. (2015) Nature 520(7548), 511-517. PDB: 3J9P.
8) Pryor, E. E., et al. (2013) Science 339(6127), 1600-1604. PDB: 4IL3.
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