Our premixed solutions.
The perfect solution.

In October, 2015, we launched our premixed DDM:CHS solution in response to customer demand, and increasing use of this mixture in the literature. The advantages of this premixed solution compared to making it in the lab are a strict QC protocol which ensures exact concentrations of each component and no lot to lot variability. This is in addition to the huge time savings!

Since the initial launch of this solution, we have offered a number of other premixed detergent and lipid mixtures all aimed at standardizing the reagents you use in the lab, saving you time, and increasing your productivity. These include our premixed DMPC:CHAPSO and DMPC:DHPC bicelle mixtures for crystallization, and our premixed monoolein:cholesterol for LCP crystallization we launched last month. Due to the increasing use in the literature and requests from customers like you, this month we’re excited to launch our new premixed 5% : 0.5% LMNG (NG310) : CHS (CH210) solution.
 
In recent years, the use of LMNG mixed with CHS has been gaining in popularity for the solubilization, purification, and structure determination of membrane proteins. In 2017, LMNG mixed with CHS was the third most commonly used detergent for solubilization of membrane proteins from their native lipid environment (used in 10% of the unique membrane protein structures) behind DDM (29%) and DDM+CHS (21%) (for a full recap of the most commonly used detergents in 2017, please see our February newsletter). Similarly, for membrane protein purification, LMNG + CHS was used in nearly 10% of the unique structures deposited in 2017.
 
Lastly, in 2017 there were four structures determined using LMNG+CHS as the detergent. Two of these structures were determined by Cryo-EM: The calcitonin receptor-heterotrimeric Gs protein complex (PDB: 5UZ7)⁽¹⁾, and the Cyclic-nucleotide-gated channel (PDB: 5V4S)⁽²⁾, while the other two structures were determined by X-ray crystallography using LCP as the crystallization method: The A₁ adenosine receptor (PDB: 5UEN)⁽³⁾, and the Plant MATE protein (PDB: 5XIJ)⁽⁴⁾. These LCP structures are in addition to the over 10 structures determined in recent years using LMNG+CHS as the detergent, including: The M3 muscarinic acetylcholine receptor in 2012 (PDB: 4DAJ)⁽⁵⁾, the GPR40 free fatty-acid receptor 1 in 2014 (PDB: 4PHU)⁽⁶⁾, and the Human σ₁ receptor in 2016 (PDB: 5HK1)⁽⁷⁾. These structures show that the use of LMNG+CHS is applicable to multiple classes of membrane proteins and structure determination methods.
 
In these published structures, LMNG is typically used at a ten-fold excess over CHS. The most commonly used concentration for membrane protein solubilization is 1% LMNG : 0.1% CHS. For membrane protein purification and structure determination, the commonly used concentration is 0.01% LMNG : 0.001% CHS. Our new premixed LMNG : CHS solution (NG310 – CH210) is supplied at a 5% : 0.5% concentration, giving you the ability to dilute this solution to whatever working concentration you need for solubilization, purification, or structure determination.
 
To find out more information about this new premixed solution, visit the product page here, or contact customer service. Do you have a detergent mixture that you would like us to make? Contact us and we’ll be happy to discuss your project with you.