● Formulation components – Dodecyl Maltoside (D310) and Cholesteryl Hemisuccinate (CH210)
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Formulation -
o 10:1 Dodecyl Maltoside to Cholesteryl Hemisuccinate (CH210) in deionized water
o Final concentration 10% Dodecyl Maltoside (D310) to 1% Cholesteryl Hemisuccinate (CH210)
o Typical working concentrations: 1% DDM, 0.1% CHS for protein extraction and 0.1% DDM, 0.01% CHS for protein purification.
●
Appearance – Clear liquid
●
Quality Assurance Tested – tested to 5 freeze thaw cycles
Below is a sample of structures solved with DDM/CHS(1):
Protein |
Family |
Extraction Detergent |
Purification Detergent |
Gel Filtration Detergent |
PDB ID (linked) |
ZMPSTE24 |
Intramembrane protease |
DDM + CHS |
DDM + CHS |
DDM + CHS |
4AW6 |
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|
N/OFQ receptor |
GPCR |
DDM + CHS |
DDM + CHS |
DDM + CHS |
4EA3 |
|
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|
|
CCR5 |
GPCR |
DDM + CHS |
DDM + CHS |
DDM + CHS |
4MBS |
|
|
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|
|
PAR1 |
GPCR |
DDM + CHS |
DDM + CHS |
DDM + CHS |
3VW7 |
|
|
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|
|
|
5-HT1B/2B serotonin receptor |
GPCR |
DDM + CHS |
DDM + CHS |
DDM + CHS |
4IAQ |
|
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|
|
Smoothened receptor |
GPCR |
DDM + CHS |
DDM + CHS |
DDM + CHS |
4JKV |
|
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|
|
|
|
Glucagon receptor |
GPCR |
DDM + CHS |
DDM + CHS |
DDM + CHS |
4L6R |
|
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|
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Metabotropic glutamate receptor 1 |
GPCR |
DDM + CHS |
DDM + CHS |
DDM + CHS |
4OR2 |
Citation:
1. He, Y., Wang, K., and Yan, N. (2014)
Protein Cell 5(9), 658-672.
Citation(s):
- Shaw K et al. 2009 Anal Chim Acta 652 231–3
- Mauk M et al. 2014 Methods Mol Biology 1256 15–40
- Lim J et al. 2018 J Assist Reprod Gen 35 637–41
- Zhang L et al. 2015 Biomicrofluidics 9 064118
- Miyaoka Y et al. 2016 Sci Rep-uk 6 23549
- Takamura Y et al. PLoS ONE 13 e0201851
No data available.