D310-CH210 - 10:1 DDM/CHS Pre-Made Solution

● Formulation components – Dodecyl Maltoside (D310) and Cholesteryl Hemisuccinate (CH210)
● Formulation - 
               o  10:1 Dodecyl Maltoside to Cholesteryl Hemisuccinate (CH210) in deionized water               
               o   Final concentration 10% Dodecyl Maltoside (D310) to 1% Cholesteryl Hemisuccinate (CH210)
               o   Typical working concentrations: 1% DDM, 0.1% CHS for protein extraction and 0.1% DDM, 0.01%                                         CHS for protein purification.
● Appearance – Clear liquid
● Quality Assurance Tested – tested to 5 freeze thaw cycles



Below is a sample of structures solved with DDM/CHS⁽¹⁾:  

Protein	Family	Extraction Detergent	Purification Detergent	Gel Filtration Detergent	PDB ID (linked)
ZMPSTE24	Intramembrane protease	DDM + CHS	DDM + CHS	DDM + CHS	4AW6
N/OFQ receptor	GPCR	DDM + CHS	DDM + CHS	DDM + CHS	4EA3
CCR5	GPCR	DDM + CHS	DDM + CHS	DDM + CHS	4MBS
PAR1	GPCR	DDM + CHS	DDM + CHS	DDM + CHS	3VW7
5-HT1B/2B serotonin receptor	GPCR	DDM + CHS	DDM + CHS	DDM + CHS	4IAQ
Smoothened receptor	GPCR	DDM + CHS	DDM + CHS	DDM + CHS	4JKV
Glucagon receptor	GPCR	DDM + CHS	DDM + CHS	DDM + CHS	4L6R
Metabotropic glutamate receptor 1	GPCR	DDM + CHS	DDM + CHS	DDM + CHS	4OR2

Citation:
1. He, Y., Wang, K., and Yan, N. (2014) Protein Cell 5(9), 658-672.
Citation(s):

1. Shaw K et al. 2009 Anal Chim Acta 652 231–3
2. Mauk M et al. 2014 Methods Mol Biology 1256 15–40
3. Lim J et al. 2018 J Assist Reprod Gen 35 637–41
4. Zhang L et al. 2015 Biomicrofluidics 9 064118
5. Miyaoka Y et al. 2016 Sci Rep-uk 6 23549
6. Takamura Y et al. PLoS ONE 13 e0201851

MSDS

No data available.