Molecular Formula: 3:1:1 POPC : POPE : POPG
Storage Conditions: -20°C
Solubility: Chloroform
Appearance: Dried Lipid Film
The most commonly used lipid mixture for nanodisc reconstitution is POPC : POPE : POPG in a 3:1:1 ratio which is typically used at a concentration of 0.1 mg/ml. To facilitate reconstitution into lipid nanodiscs and saposin/lipid nanoparticles, we are excited to offer our 3:1:1 POPC:POPE:POPG lipid mix. This mix is provided as a dried lipid film stored under argon. To use, simply solubilize the lipid mix in a detergent containing buffer and use in your experiments.
Examples of Cryo-EM structures determined using this phospholipid mix include:
- The NOMPC channel (PDB: 5VKQ)(1)
- The TRPML1 channel (PDB: 5WPV)(2)
- The TRPM4 channel (PDB: 6BCJ)(3)
- The Kv1.2–2.1 paddle chimera channel (PDB: 6EBK)(4)
Need another lipid mixture for your reconstitution experiments? Just ask!
References:
- Jin P, et al. (2017) Nature 547(7661):118-122
- Chen Q, et al. (2017) Nature 550(7676): 415-418
- Guo J, et al. (2017) Nature 552(7684): 205-209
- Matthies D. et al. (2018) Elife e37558. doi:10.7554/eLife.37558
No data available.
No data available.