INTRODUCTION AND PURPOSE OF USE: The COVID-19 system is a quantitative test that allows the quantification, by means of Real Time PCR, of the N region (nucleocapsid phosphoprotein) of novel Coronavirus (COVID-19).
The Procedure allows the detection of the RNA target by means a retro-amplification reaction in a microplate.
The analysis of the results is made using a Real Time PCR analyzer instrument (thermal cycler integrated with a system for fluorescence detection).
SAMPLES AND STORAGE: The quanty COVID-19 kit must be used with extracted RNA from biological samples: Nasopharyngeal/oropharyngeal swabs, sputum and serum. Collected samples must be shipped and stored at +2 - +8°C and used within 3 days.
Citation(s):
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- Mauk M et al. 2014 Methods Mol Biology 1256 15–40
- Lim J et al. 2018 J Assist Reprod Gen 35 637–41
- Zhang L et al. 2015 Biomicrofluidics 9 064118
- Miyaoka Y et al. 2016 Sci Rep-uk 6 23549
- Takamura Y et al. PLoS ONE 13 e0201851
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