Affinity purification of monoclonal antibodies has been largely confined to the use of Protein A and Protein G chromatography. The immobilized Protein A resin is designed for simple, one-step and rapid antibody purification from serum, ascites and tissue culture supernatant such as those derived from static cultures and bioreactors. Antibody samples purified using this affinity resin may be used in a wide range of laboratory procedures such as 1D or 2D polyacrylamide gel electrophoresis, Western blotting, ELISA etc. The antibodies are sufficiently pure for radiolabelling, conjugations (for example fluorescein) or preparation of immuno-affinity columns.
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